Scientific Publications

6 month serologic response to the Pfizer-BioNTech COVID-19 vaccine among healthcare workers

Healthcare workers (HCWs) were among the first group of people vaccinated with the Pfizer-BioNTech Covid-19 vaccine (BNT162b2). Characterization of the kinetics of antibody response to vaccination is important to devise future vaccination strategies. To better characterize the antibody response to BNT162b2, we analyzed the kinetics of IgG and IgM antibody response to 5 different SARS-CoV-2 epitopes over a period of 6 months.

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Cell Reports Methods, Rapid detection of an Ebola biomarker with optical microring resonators

Qavi et al. describe a method using microring resonator sensors to detect Ebola virus (EBOV) soluble glycoprotein (sGP) in serum. sGP is a promising biomarker for the prognosis of EBOV infection and, coupled with this rapid detection method, could lead to a faster diagnosis.

Highlights

• sGP is a unique diagnostic marker of Ebola virus infection
• Microring resonators can detect EBOV and SUDV sGP at low ng/mL concentrations
• Our sandwich immunoassay design enables the detection of antigens from serum
• Multiplex chip has flexible design, technical replicates, and integrated controls

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Pre-COVID-19 Immunity to Common Cold Human Coronaviruses Induces a Recall-Type IgG Response to SARS-CoV-2 Antigens Without Cross-Neutralisation

The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against de novo COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG) batches. We found that most COVID-19 patients developed anti-SARS-CoV-2 IgG antibodies before IgM. Moreover, the capacity of their IgGs to react to beta-HCoV, was present in the early sera of most patients before the appearance of anti-SARS-CoV-2 IgG. This implied that a recall-type antibody response was generated. In comparison, the patients that mounted an anti-SARS-COV2 IgM response, prior to IgG responses had lower titres of anti-beta-HCoV IgG antibodies. This indicated that pre-existing immunity to beta-HCoV was conducive to the generation of memory type responses to SARS-COV-2. Finally, we also found that pre-COVID-19-era sera and IVIG cross-reacted with SARS-CoV-2 antigens without neutralising SARS-CoV-2 infectivity in vitro. Put together, these results indicate that whilst pre-existing immunity to HCoV is responsible for recall-type IgG responses to SARS-CoV-2, it does not lead to cross-protection against COVID-19. Front. Immunol., 11 February 2022

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BNT162b2 vaccine-induced humoral and cellular responses against SARS-CoV-2 variants in systemic lupus erythematosus

Correspondence to Dr Zahir Amoura, Assistance Publique–Hôpitaux de Paris, Groupement Hospitalier Pitié–Salpêtrière, French National Reference Center for Systemic Lupus Erythematosus, Antiphospholipid Antibody Syndrome and Other Autoimmune Disorders, Service de Médecine Interne 2, Institut E3M, Sorbonne Université, Paris, 75006 Île-de-France, France. October 3rd, 2021

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Target specific serologic analysis of COVID-19 convalescent plasma

Sachie Ikegami,Robert C. Benirschke,Hossein Fakhrai-Rad,Mohammad H. Motamedi,Rick Hockett,Sean David,Hong Kee Lee,Jason Kang,Thomas J. Gniadek. April 28, 2021

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IgA dominates the early neutralizing antibody response to SARS-CoV-2

Delphine Sterlin, Alexis Mathian, Audrey Mohr, François Anna, Laetitia Claër, Paul Quentric , Jehane Fadlallah , Hervé Devilliers. 07 Dec 2020

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Anti-MDA5 juvenile idiopathic inflammatory myopathy: a specific subgroup defined by differentially enhanced interferon-α signalling

Isabelle Melki, Hervé Devilliers, Cyril Gitiaux, Vincent Bondet, Darragh Duffy, Jean-Luc Charuel , Makoto Miyar, Plamen Bokov, Ahmed Kheniche, Theresa Kwon.  Nov 2019

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Détection des anticorps anti-MDA5 par une nouvelle technique simple et rapide

Y.Uzunhan, R.Chieze, J.L.Charuel, S.Mudumba, H.Nunes, R.Burlingam , M.Miyara. Jan 2019

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Detection in whole blood of autoantibodies for the diagnosis of connective tissue diseases in near patient testing condition

Makoto Miyara , Jean-Luc Charuel, Sasi Mudumba, Alice Wu, Pascale Ghillani-Dalbin, Zahir Amoura , Rufus W. Burlingame , Lucile Musset. Aug 2018

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Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time.

Sasi Mudumba, Sophia de Alba, Randy Romero, Carli Cherwien, Alice Wu, Jue Wang, Martin A. Gleeson, Muzammil Iqbal, Rufus W. Burlingame. J. Immunol. Methods (2017).

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Emerging Biosensing Approaches for microRNA Analysis.

Graybill RM, Bailey RC. Anal Chem, 2016 Jan 5;88(1):431-50.

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Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform

Enrique Valera, Winnie W. Shia, and Ryan C. Bailey. Clinical Biochemistry.  2016, Volume 49, Issues 1-2, Pages 121-126.

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Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays

James H. Wade, Aurora T. Alsop, Nicholas R. Vertin, Hongwei Yang, Mark D. Johnson, and Ryan C. Bailey. ACS Cent. Sci. 2015, 1 (7):374-382.

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Magnetically-actuated, bead-enhanced silicon photonic immunosensor.

Valera E, McClellan MS, Bailey RC. Anal Methods. 2015 Oct 21;7(20):8539-8544.

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High-Q optical sensors for chemical and biological analysis.

Luchansky MS, and Bailey RC. Anal Chem. 2012, 84(2):793-821.

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An organophosphonate strategy for functionalizing silicon photonic biosensors.

Shang J, Cheng F, Dubey M, Kaplan JM, Rawal M, Jiang X, Newburg DS, Sullivan PA, Andrade RB, Ratner DM. Langmuir. 2012, 28(6):3338-44.

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Label-free virus detection using silicon photonic microring resonators.

McClellan MS, Domier LL, Bailey RC. Biosens Bioelectron. 2012, 31(1):388-92.

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Label-free, multiplexed biomolecular analysis using arrays of silicon photonic microring resonators.

Washburn A.L., Luchansky MS., McClellan MS., and Bailey RC. 2012, Procedia Eng.

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Isothermal Discrimination of Single-Nucleotide Polymorphisms via Kinetic Desorption and Label-Free Detection of DNA using Silicon Photonic Microring Resonator Arrays.

Qavi AJ., Mysz TM., and Bailey RC. Anal Chem, 2011, 83(17):6827-33.

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Anti-DNA:RNA Antibodies and Silicon Photonic Microring Resonators: Increased Sensitivity for Multiplexed microRNA Detection.

Qavi AJ, Kindt JT, Gleeson MA, and Bailey RC, Anal Chem, 2011, 83, 5949-56.

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Multiplexed inkjet functionalization of silicon photonic biosensors.

Kirk JT, Fridley GE, Chamberlain JW, Christensen ED, Hochberg M, Ratner DM. Lab Chip. 2011, 11(7):1372-7.

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Label-Free, Multiplexed Determination of Aptamer and Antibody Capture Agent Binding Affinities Using Silicon Photonic Microring Resonator Arrays and Implications for Sensitive Biomolecule Detection.

Byeon, J.-Y and Bailey RC, Analyst, 2011, 136(17):3430-3.

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Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads.

Luchansky MS, Washburn AL, McClellan MS, and Bailey RC, Lab Chip, 2011, 11, 2042-2044.

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DNA-Encoding to Improve Performance and Allow Parallel Evaluation of the Binding Characteristics of Multiple Antibodies in a Surface-Bound Immunoassay Format.

Washburn A.L, Gomez J, and Bailey R.C. Anal Chem, 2011, 83, 3572-3580.

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Silicon photonic microring resonator arrays for scalable and multiplexable bioanalysis.

Luchansky M.S., Washburn AL, Qavi A.J, Kindt JT, McClellan MS, and Bailey RC, Proc SPIE, 2011, 7888, 788802.

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Photonics-on-a-Chip: Recent Advances in Integrated Waveguides as Enabling Detection Elements for Real-World. Lab-on-a-Chip Biosens Applicatios.

Washburn AL,A and Bailey RC. Analyst, 2011, 136, 227-236.

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Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators.

Byeon J-Y, and Bailey RC. Analyst, 2011, 136, 3430-3433.

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Sizing up the Future of MicroRNA Analysis.

Qavi AJ, Kindt JT, and Bailey RC, Anal Bioanal Chem, 2010, 398, 2535-2549.

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Efficient Bioconjugation of Protein Capture Agents to Biosensor Surfaces Using Aniline-Catalyzed Hydrazone Ligation.

Byeon J.-Y, Limpoco FT, and Bailey RC, Langmuir, 2010, 26, 15430-15435.

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Label-Free Biosensor Arrays based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation.

Iqbal M., Gleeson M.A., Spaugh B., Tybor F., Gunn W.G, Hochberg M, Baehr-Jones T, Bailey R.C, and. Gunn LC. J. Sel. Topics Quantum Electron., 2010, 16, 654-661.

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Quantitative Multiplexed Detection of Five Protein Biomarkers Using Arrays of Silicon Photonic Microring Resonators.

Washburn AL, Luchansky MS, Bowman AL, and Bailey RC, Anal. Chem., 2010, 82, 69-72.

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Characterization of the Evanescent Field Profile and Bound Mass Sensitivity of a Label-Free Silicon Photonic Microring Resonator Biosensing Platform.

Luchansky MS, Washburn AL, Martin TA, Iqbal M, Gunn LC, and Bailey RC, Biosens Bioelectron, 2010, 26(4):1283-91.

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Multiplexed Detection and Label-Free Quantitation of MicroRNAs Using Arrays of Silicon Photonic Microring Resonators.

Qavi A.J. and Bailey R.C. Angew. Chem. Intl. Ed., 2010, 49, 4608-4611.

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Silicon Photonic Microring Resonators for Quantitative Cytokine Detection and T-cell Secretion Analysis.

Luchansky MS, and Bailey RC, Anal. Chem., 2010, 82, 1975-1981.

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New Multiparameter Bioanalytical Technologies for Applications in Personalized Medicine, Drug Discovery, and Fundamental Biology.

Bailey RC, Bioanal, 2009, 1, 1043-1047.

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Label-Free Technologies for Multiparameter Biological Analysis.

Qavi AJ, Byeon J-Y, Washburn AL, and Bailey RC, Anal Bioanal Chem, 2009, 394, 121-135.

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Label-free quantitation of a cancer biomarker in complex media using silicon photonic microring resonators.

Washburn AL, Gunn LC, Bailey RC. Anal Chem. 2009, 81(22):9499-506.

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