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The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against de novo COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG) batches. We found that most COVID-19 patients developed anti-SARS-CoV-2 IgG antibodies before IgM. Moreover, the capacity of their IgGs to react to beta-HCoV, was present in the early sera of most patients before the appearance of anti-SARS-CoV-2 IgG. This implied that a recall-type antibody response was generated. In comparison, the patients that mounted an anti-SARS-COV2 IgM response, prior to IgG responses had lower titres of anti-beta-HCoV IgG antibodies. This indicated that pre-existing immunity to beta-HCoV was conducive to the generation of memory type responses to SARS-COV-2. Finally, we also found that pre-COVID-19-era sera and IVIG cross-reacted with SARS-CoV-2 antigens without neutralising SARS-CoV-2 infectivity in vitro. Put together, these results indicate that whilst pre-existing immunity to HCoV is responsible for recall-type IgG responses to SARS-CoV-2, it does not lead to cross-protection against COVID-19. Front. Immunol., 11 February 2022
Correspondence to Dr Zahir Amoura, Assistance Publique–Hôpitaux de Paris, Groupement Hospitalier Pitié–Salpêtrière, French National Reference Center for Systemic Lupus Erythematosus, Antiphospholipid Antibody Syndrome and Other Autoimmune Disorders, Service de Médecine Interne 2, Institut E3M, Sorbonne Université, Paris, 75006 Île-de-France, France. October 3rd, 2021
Target specific serologic analysis of COVID-19 convalescent plasma
Sachie Ikegami,Robert C. Benirschke,Hossein Fakhrai-Rad,Mohammad H. Motamedi,Rick Hockett,Sean David,Hong Kee Lee,Jason Kang,Thomas J. Gniadek. April 28, 2021
IgA dominates the early neutralizing antibody response to SARS-CoV-2
Delphine Sterlin, Alexis Mathian, Audrey Mohr, François Anna, Laetitia Claër, Paul Quentric , Jehane Fadlallah , Hervé Devilliers. 07 Dec 2020
Isabelle Melki, Hervé Devilliers, Cyril Gitiaux, Vincent Bondet, Darragh Duffy, Jean-Luc Charuel , Makoto Miyar, Plamen Bokov, Ahmed Kheniche, Theresa Kwon. Nov 2019
Détection des anticorps anti-MDA5 par une nouvelle technique simple et rapide
Y.Uzunhan, R.Chieze, J.L.Charuel, S.Mudumba, H.Nunes, R.Burlingam , M.Miyara. Jan 2019
Makoto Miyara , Jean-Luc Charuel, Sasi Mudumba, Alice Wu, Pascale Ghillani-Dalbin, Zahir Amoura , Rufus W. Burlingame , Lucile Musset. Aug 2018
Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time.
Sasi Mudumba, Sophia de Alba, Randy Romero, Carli Cherwien, Alice Wu, Jue Wang, Martin A. Gleeson, Muzammil Iqbal, Rufus W. Burlingame. J. Immunol. Methods (2017).
Emerging Biosensing Approaches for microRNA Analysis.
Graybill RM, Bailey RC. Anal Chem, 2016 Jan 5;88(1):431-50.
Enrique Valera, Winnie W. Shia, and Ryan C. Bailey. Clinical Biochemistry. 2016, Volume 49, Issues 1-2, Pages 121-126.
Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays
James H. Wade, Aurora T. Alsop, Nicholas R. Vertin, Hongwei Yang, Mark D. Johnson, and Ryan C. Bailey. ACS Cent. Sci. 2015, 1 (7):374-382.
Magnetically-actuated, bead-enhanced silicon photonic immunosensor.
Valera E, McClellan MS, Bailey RC. Anal Methods. 2015 Oct 21;7(20):8539-8544.
High-Q optical sensors for chemical and biological analysis.
Luchansky MS, and Bailey RC. Anal Chem. 2012, 84(2):793-821.
An organophosphonate strategy for functionalizing silicon photonic biosensors.
Shang J, Cheng F, Dubey M, Kaplan JM, Rawal M, Jiang X, Newburg DS, Sullivan PA, Andrade RB, Ratner DM. Langmuir. 2012, 28(6):3338-44.
Label-free virus detection using silicon photonic microring resonators.
McClellan MS, Domier LL, Bailey RC. Biosens Bioelectron. 2012, 31(1):388-92.
Label-free, multiplexed biomolecular analysis using arrays of silicon photonic microring resonators.
Washburn A.L., Luchansky MS., McClellan MS., and Bailey RC. 2012, Procedia Eng.
Qavi AJ., Mysz TM., and Bailey RC. Anal Chem, 2011, 83(17):6827-33.
Qavi AJ, Kindt JT, Gleeson MA, and Bailey RC, Anal Chem, 2011, 83, 5949-56.
Multiplexed inkjet functionalization of silicon photonic biosensors.
Kirk JT, Fridley GE, Chamberlain JW, Christensen ED, Hochberg M, Ratner DM. Lab Chip. 2011, 11(7):1372-7.
Byeon, J.-Y and Bailey RC, Analyst, 2011, 136(17):3430-3.
Luchansky MS, Washburn AL, McClellan MS, and Bailey RC, Lab Chip, 2011, 11, 2042-2044.
Washburn A.L, Gomez J, and Bailey R.C. Anal Chem, 2011, 83, 3572-3580.
Silicon photonic microring resonator arrays for scalable and multiplexable bioanalysis.
Luchansky M.S., Washburn AL, Qavi A.J, Kindt JT, McClellan MS, and Bailey RC, Proc SPIE, 2011, 7888, 788802.
Washburn AL,A and Bailey RC. Analyst, 2011, 136, 227-236.
Byeon J-Y, and Bailey RC. Analyst, 2011, 136, 3430-3433.
Sizing up the Future of MicroRNA Analysis.
Qavi AJ, Kindt JT, and Bailey RC, Anal Bioanal Chem, 2010, 398, 2535-2549.
Byeon J.-Y, Limpoco FT, and Bailey RC, Langmuir, 2010, 26, 15430-15435.
Iqbal M., Gleeson M.A., Spaugh B., Tybor F., Gunn W.G, Hochberg M, Baehr-Jones T, Bailey R.C, and. Gunn LC. J. Sel. Topics Quantum Electron., 2010, 16, 654-661.
Washburn AL, Luchansky MS, Bowman AL, and Bailey RC, Anal. Chem., 2010, 82, 69-72.
Luchansky MS, Washburn AL, Martin TA, Iqbal M, Gunn LC, and Bailey RC, Biosens Bioelectron, 2010, 26(4):1283-91.
Qavi A.J. and Bailey R.C. Angew. Chem. Intl. Ed., 2010, 49, 4608-4611.
Luchansky MS, and Bailey RC, Anal. Chem., 2010, 82, 1975-1981.
Bailey RC, Bioanal, 2009, 1, 1043-1047.
Label-Free Technologies for Multiparameter Biological Analysis.
Qavi AJ, Byeon J-Y, Washburn AL, and Bailey RC, Anal Bioanal Chem, 2009, 394, 121-135.
Washburn AL, Gunn LC, Bailey RC. Anal Chem. 2009, 81(22):9499-506.
Connective Tissue Diseases
Novel Multiplex Measurement of Macromolecules Binding To A Solid Phase Using Photonic Microring Sensors
Applications For Autoimmunity
Immunogenicity
Detection of Isotype of Anti-Adalimumab Antibodies using a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a human anti-Adalimumab Multi-Tier Anti-Drug Antibody (MT-ADA) assay. This is a multiplex assay that determines the isotype and IgG subclass of ADA in a single run, including IgG4. The sensitivity and free drug tolerance exceed FDA guidelines.
Detection and Isotyping of Anti-Infliximab Antibodies using a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a human anti-Infliximab Multi-Tier Anti-Drug Antibody (MT-ADA) assay. This is a multiplex assay that determines the isotype and IgG subclass of ADA in a single run, including IgG4. The sensitivity and free drug tolerance exceed FDA guidelines.
Detection of Anti-Drug Antibodies in Rabbits with a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a Rabbit Multi-Tier Anti-Drug Antibody (rMT-ADA) assay. This is a multiplex assay that measures the isotype of anti-drug antibodies (ADA) in a single run. The sensitivity and free drug tolerance exceed FDA guidelines.
Connective Tissue Diseases
Systemic Lupus Erythematosus with Anti-DSF70 Antibodies
Presented at the 11th International Congress of Autoimmunity, May 16th-20th, 2018, Lisbon, Portugal.
Near Patient Detection of anti MDA5 Antibodies Using Photonic Ring Immunoassays 2017
Presented at the 2017 ATS Conference, May 24, 2017, Washington D.C.
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Presented at the 2015 ACR/ARHP Annual Meeting, November 6-11, 2015, San Francisco CA
Immunogenicity
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