Scientific Publications
Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time
Abstract Photonic ring resonance is a property of light where in certain circumstances specific wavelengths are trapped in a ring resonator. Sensors based on silicon photonic ring resonators function by detecting the interaction between light circulating inside the...
Near Patient Detection of anti MDA5 Antibodies Using Photonic Ring Immunoassays 2017
Presented at the 2017 ATS Conference, May 24, 2017, Washington D.C.
Near Patient Anti-Nuclear Antibody Multiplex Testing Using Whole Blood for the Diagnosis of Connective Tissue Diseases in a Tertiary Care Center
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Application of a Novel Anti-Nuclear Antibody Multiplex Test Using Fingerstick and Venous Whole Blood in a Rheumatology Clinic
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform
Abstract Objectives We report the development of an optical immunosensor for the detection of monocyte chemotactic protein 1 (MCP-1) in serum samples. MCP-1 is a cytokine that is an emerging biomarker for several diseases/disorders, including ischemic...
Emerging Biosensing Approaches for microRNA Analysis
Biosensor Methods miRNAs have been a focus of biosensor development over the past decade, with many micro- and nanoscale sensing technologies being applied to this class of analytes. Benefits of many of these schemes lie in their potential for diagnostics at the point...
Characterization of Anti-Nuclear Antibody (ANA) Signatures in Murine Models of Lupus Using Genalyte Maverick Technology
Presented at the 2015 ACR/ARHP Annual Meeting, November 6-11, 2015, San Francisco CA
Detection of all ADA subclass and isotypes, including monovalent IgG4, on a Multiplex Photonic Ring Immunoassay
Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays
Abstract Extracellular signaling is commonly mediated through post-translational protein modifications that propagate messages from membrane-bound receptors to ultimately regulate gene expression. Signaling cascades are ubiquitously intertwined, and a full...
Magnetically-actuated, bead-enhanced silicon photonic immunosensor
Abstract Magnetic actuation has been introduced to an optical immunosensor technology resulting in improvements in both rapidity and limit of detection for an assay quantitating low concentrations of a representative protein biomarker. For purposes of demonstration,...
Posters and Brochures
Systemic Lupus Erythematosus with Anti-DSF70 Antibodies
Presented at the 11th International Congress of Autoimmunity, May 16th-20th, 2018, Lisbon,...
Read MoreNear Patient Detection of anti MDA5 Antibodies Using Photonic Ring Immunoassays 2017
Presented at the 2017 ATS Conference, May 24, 2017, Washington D.C.
Read MoreNear Patient Anti-Nuclear Antibody Multiplex Testing Using Whole Blood for the Diagnosis of Connective Tissue Diseases in a Tertiary Care Center
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Read MoreApplication of a Novel Anti-Nuclear Antibody Multiplex Test Using Fingerstick and Venous Whole Blood in a Rheumatology Clinic
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Read More
Healthcare workers (HCWs) were among the first group of people vaccinated with the Pfizer-BioNTech Covid-19 vaccine (BNT162b2). Characterization of the kinetics of antibody response to vaccination is important to devise future vaccination strategies. To better characterize the antibody response to BNT162b2, we analyzed the kinetics of IgG and IgM antibody response to 5 different SARS-CoV-2 epitopes over a period of 6 months.
Cell Reports Methods, Rapid detection of an Ebola biomarker with optical microring resonators
Qavi et al. describe a method using microring resonator sensors to detect Ebola virus (EBOV) soluble glycoprotein (sGP) in serum. sGP is a promising biomarker for the prognosis of EBOV infection and, coupled with this rapid detection method, could lead to a faster diagnosis.
Highlights
- sGP is a unique diagnostic marker of Ebola virus infection
- Microring resonators can detect EBOV and SUDV sGP at low ng/mL concentrations
- Our sandwich immunoassay design enables the detection of antigens from serum
- Multiplex chip has flexible design, technical replicates, and integrated controls
The capacity of pre-existing immunity to human common coronaviruses (HCoV) to cross-protect against de novo COVID-19is yet unknown. In this work, we studied the sera of 175 COVID-19 patients, 76 healthy donors and 3 intravenous immunoglobulins (IVIG) batches. We found that most COVID-19 patients developed anti-SARS-CoV-2 IgG antibodies before IgM. Moreover, the capacity of their IgGs to react to beta-HCoV, was present in the early sera of most patients before the appearance of anti-SARS-CoV-2 IgG. This implied that a recall-type antibody response was generated. In comparison, the patients that mounted an anti-SARS-COV2 IgM response, prior to IgG responses had lower titres of anti-beta-HCoV IgG antibodies. This indicated that pre-existing immunity to beta-HCoV was conducive to the generation of memory type responses to SARS-COV-2. Finally, we also found that pre-COVID-19-era sera and IVIG cross-reacted with SARS-CoV-2 antigens without neutralising SARS-CoV-2 infectivity in vitro. Put together, these results indicate that whilst pre-existing immunity to HCoV is responsible for recall-type IgG responses to SARS-CoV-2, it does not lead to cross-protection against COVID-19. Front. Immunol., 11 February 2022
Correspondence to Dr Zahir Amoura, Assistance Publique–Hôpitaux de Paris, Groupement Hospitalier Pitié–Salpêtrière, French National Reference Center for Systemic Lupus Erythematosus, Antiphospholipid Antibody Syndrome and Other Autoimmune Disorders, Service de Médecine Interne 2, Institut E3M, Sorbonne Université, Paris, 75006 Île-de-France, France. October 3rd, 2021
Target specific serologic analysis of COVID-19 convalescent plasma
Sachie Ikegami,Robert C. Benirschke,Hossein Fakhrai-Rad,Mohammad H. Motamedi,Rick Hockett,Sean David,Hong Kee Lee,Jason Kang,Thomas J. Gniadek. April 28, 2021
IgA dominates the early neutralizing antibody response to SARS-CoV-2
Delphine Sterlin, Alexis Mathian, Audrey Mohr, François Anna, Laetitia Claër, Paul Quentric , Jehane Fadlallah , Hervé Devilliers. 07 Dec 2020
Isabelle Melki, Hervé Devilliers, Cyril Gitiaux, Vincent Bondet, Darragh Duffy, Jean-Luc Charuel , Makoto Miyar, Plamen Bokov, Ahmed Kheniche, Theresa Kwon. Nov 2019
Détection des anticorps anti-MDA5 par une nouvelle technique simple et rapide
Y.Uzunhan, R.Chieze, J.L.Charuel, S.Mudumba, H.Nunes, R.Burlingam , M.Miyara. Jan 2019
Makoto Miyara , Jean-Luc Charuel, Sasi Mudumba, Alice Wu, Pascale Ghillani-Dalbin, Zahir Amoura , Rufus W. Burlingame , Lucile Musset. Aug 2018
Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time.
Sasi Mudumba, Sophia de Alba, Randy Romero, Carli Cherwien, Alice Wu, Jue Wang, Martin A. Gleeson, Muzammil Iqbal, Rufus W. Burlingame. J. Immunol. Methods (2017).
Emerging Biosensing Approaches for microRNA Analysis.
Graybill RM, Bailey RC. Anal Chem, 2016 Jan 5;88(1):431-50.
Enrique Valera, Winnie W. Shia, and Ryan C. Bailey. Clinical Biochemistry. 2016, Volume 49, Issues 1-2, Pages 121-126.
Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays
James H. Wade, Aurora T. Alsop, Nicholas R. Vertin, Hongwei Yang, Mark D. Johnson, and Ryan C. Bailey. ACS Cent. Sci. 2015, 1 (7):374-382.
Magnetically-actuated, bead-enhanced silicon photonic immunosensor.
Valera E, McClellan MS, Bailey RC. Anal Methods. 2015 Oct 21;7(20):8539-8544.
High-Q optical sensors for chemical and biological analysis.
Luchansky MS, and Bailey RC. Anal Chem. 2012, 84(2):793-821.
An organophosphonate strategy for functionalizing silicon photonic biosensors.
Shang J, Cheng F, Dubey M, Kaplan JM, Rawal M, Jiang X, Newburg DS, Sullivan PA, Andrade RB, Ratner DM. Langmuir. 2012, 28(6):3338-44.
Label-free virus detection using silicon photonic microring resonators.
McClellan MS, Domier LL, Bailey RC. Biosens Bioelectron. 2012, 31(1):388-92.
Label-free, multiplexed biomolecular analysis using arrays of silicon photonic microring resonators.
Washburn A.L., Luchansky MS., McClellan MS., and Bailey RC. 2012, Procedia Eng.
Qavi AJ., Mysz TM., and Bailey RC. Anal Chem, 2011, 83(17):6827-33.
Qavi AJ, Kindt JT, Gleeson MA, and Bailey RC, Anal Chem, 2011, 83, 5949-56.
Multiplexed inkjet functionalization of silicon photonic biosensors.
Kirk JT, Fridley GE, Chamberlain JW, Christensen ED, Hochberg M, Ratner DM. Lab Chip. 2011, 11(7):1372-7.
Byeon, J.-Y and Bailey RC, Analyst, 2011, 136(17):3430-3.
Luchansky MS, Washburn AL, McClellan MS, and Bailey RC, Lab Chip, 2011, 11, 2042-2044.
Washburn A.L, Gomez J, and Bailey R.C. Anal Chem, 2011, 83, 3572-3580.
Silicon photonic microring resonator arrays for scalable and multiplexable bioanalysis.
Luchansky M.S., Washburn AL, Qavi A.J, Kindt JT, McClellan MS, and Bailey RC, Proc SPIE, 2011, 7888, 788802.
Washburn AL,A and Bailey RC. Analyst, 2011, 136, 227-236.
Byeon J-Y, and Bailey RC. Analyst, 2011, 136, 3430-3433.
Sizing up the Future of MicroRNA Analysis.
Qavi AJ, Kindt JT, and Bailey RC, Anal Bioanal Chem, 2010, 398, 2535-2549.
Byeon J.-Y, Limpoco FT, and Bailey RC, Langmuir, 2010, 26, 15430-15435.
Iqbal M., Gleeson M.A., Spaugh B., Tybor F., Gunn W.G, Hochberg M, Baehr-Jones T, Bailey R.C, and. Gunn LC. J. Sel. Topics Quantum Electron., 2010, 16, 654-661.
Washburn AL, Luchansky MS, Bowman AL, and Bailey RC, Anal. Chem., 2010, 82, 69-72.
Luchansky MS, Washburn AL, Martin TA, Iqbal M, Gunn LC, and Bailey RC, Biosens Bioelectron, 2010, 26(4):1283-91.
Qavi A.J. and Bailey R.C. Angew. Chem. Intl. Ed., 2010, 49, 4608-4611.
Luchansky MS, and Bailey RC, Anal. Chem., 2010, 82, 1975-1981.
Bailey RC, Bioanal, 2009, 1, 1043-1047.
Label-Free Technologies for Multiparameter Biological Analysis.
Qavi AJ, Byeon J-Y, Washburn AL, and Bailey RC, Anal Bioanal Chem, 2009, 394, 121-135.
Washburn AL, Gunn LC, Bailey RC. Anal Chem. 2009, 81(22):9499-506.
Connective Tissue Diseases
Novel Multiplex Measurement of Macromolecules Binding To A Solid Phase Using Photonic Microring Sensors
Applications For Autoimmunity
Immunogenicity
Detection of Isotype of Anti-Adalimumab Antibodies using a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a human anti-Adalimumab Multi-Tier Anti-Drug Antibody (MT-ADA) assay. This is a multiplex assay that determines the isotype and IgG subclass of ADA in a single run, including IgG4. The sensitivity and free drug tolerance exceed FDA guidelines.
Detection and Isotyping of Anti-Infliximab Antibodies using a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a human anti-Infliximab Multi-Tier Anti-Drug Antibody (MT-ADA) assay. This is a multiplex assay that determines the isotype and IgG subclass of ADA in a single run, including IgG4. The sensitivity and free drug tolerance exceed FDA guidelines.
Detection of Anti-Drug Antibodies in Rabbits with a Multiplex Photonic Ring Immunoassay
The present study shows the performance characteristics of a Rabbit Multi-Tier Anti-Drug Antibody (rMT-ADA) assay. This is a multiplex assay that measures the isotype of anti-drug antibodies (ADA) in a single run. The sensitivity and free drug tolerance exceed FDA guidelines.
Connective Tissue Diseases
Systemic Lupus Erythematosus with Anti-DSF70 Antibodies
Presented at the 11th International Congress of Autoimmunity, May 16th-20th, 2018, Lisbon, Portugal.
Near Patient Detection of anti MDA5 Antibodies Using Photonic Ring Immunoassays 2017
Presented at the 2017 ATS Conference, May 24, 2017, Washington D.C.
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Presented at the 2016 ACR/ARHP Annual Meeting, November 11-15, 2016, Washington D.C.
Presented at the 2015 ACR/ARHP Annual Meeting, November 6-11, 2015, San Francisco CA
Immunogenicity
Are you sure your ELISA isn’t missing something?
